Describe a protocol for isolating mitochondria from rat liver
and for estimating the purity of the...
80.2K
Verified Solution
Link Copied!
Question
Biology
Describe a protocol for isolating mitochondria from rat liverand for estimating the purity of the preparation.
Answer & Explanation
Solved by verified expert
4.5 Ratings (978 Votes)
Preparation of isolation buffer Isolation buffer consists of 4099 grams of 225mM Mannitol 3567 grams 75mM sucrose 00744 grams of 02mM EDTA being dissolved in 1000 ml of water Isolation protocol 1 Sacrifice the rat and dissect the liver out and put it in isolation medium icecold 2 Use 15 g of the tissue for the
See Answer
Get Answers to Unlimited Questions
Join us to gain access to millions of questions and expert answers. Enjoy exclusive benefits tailored just for you!
Membership Benefits:
Unlimited Question Access with detailed Answers
Zin AI - 3 Million Words
10 Dall-E 3 Images
20 Plot Generations
Conversation with Dialogue Memory
No Ads, Ever!
Access to Our Best AI Platform: Flex AI - Your personal assistant for all your inquiries!
