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Describe how you can use PCR to obtain a gene fragment X that can be cloned...

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Biology

Describe how you can use PCR to obtain a gene fragment X thatcan be cloned into a
plasmid vector for expression of the recombinant protein. Includein your description
how the PCR reaction will be performed, how the amplicon will beanalyzed, the use of
restriction enzymes, how cloning will be achieved, how you willknow that you have a
recombinant containing the insert gene fragment X and how you willdetect the
recombinant protein.

Answer & Explanation Solved by verified expert
4.4 Ratings (945 Votes)
We will clone the gene in the following steps1 amplification of gene if interstFirst we will amplify the gene of interst using specific primersof the gene of interst We will design the priners in such a waythat they must contain the restriction enzyme site present in theplasmid in which we have to    See Answer
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