Describe the 5 events that convert an mRNA into a cDNA.
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Biology
Describe the 5 events that convert an mRNA into a cDNA.
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Conversion of mRNA to cDNA involve following steps which are as follows 1 Isolation of mRNA A family of mRNA is present in the cells and tissues It is isolated from the cells 2 Reverse transcription The family of mRNAs in solution is treated with oligo d T primer the enzyme reverse transcriptase and four types of deoxyribonucleotides The temperature is maintained at 55 degree
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