Describe these two techniques: co-immunoprecipitation and
sandwich ELISA. For each technique, be sure to explain its...
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Biology
Describe these two techniques: co-immunoprecipitation andsandwich ELISA. For each technique, be sure to explain its purposeand describe the main steps involved in carrying out thetechnique.
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Coimmunoprecipitation Coimmunoprecipitation coIP is a technique to identify physiologically relevant proteinprotein interactions by using target proteinspecific antibodies to indirectly capture proteins that are bound to a specific target protein The general steps are as follows 1 Lyse your Cells 2 Add your Antibody 3 Add the Protein AG Beads 4 Incubate 5 Collect 6 Wash the Beads 7 Elute your Proteins 8 Detect your Proteins 9 Study the Interaction With Surface Plasmon Resonance
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