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DNA EXTRACTION & PURITY PROTOCOL Add a small piece of peeled banana (2-3 cm) and 12mL extraction...

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Biology

DNA EXTRACTION & PURITY PROTOCOL

  1. Add a small piece of peeled banana (2-3 cm) and 12mLextraction buffer (NaCl & Sodium Citrate) into the beaker.This buffer contains salts necessary for the precipitation ofthe DNA. The ions in the solution make it more polar facilitatingthe dissolving of the DNA.
  2. Add 3mL detergent into the beaker. This will breakopen the cells and dissolve the DNA. Detergent breaks up the lipidsof the plasma and nuclear membranes

Questions:

a. Why does a more polar solution make DNA more likely todissolve?

b. How does detergent break up lipids in a membrane?

Answer & Explanation Solved by verified expert
3.7 Ratings (673 Votes)
a The solubility of DNA depends upon the polarity of the solution Higher the polarity more will be the solubility This is because of the fact that the DNA is a polar molecule Its sugarphosphate backbone is highly charged hence    See Answer
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