Due to system limitations you are attempting to clone a smallbacterial gene using a poorly characterised bacterial plasmid. Youknow that the plasmid contains only two drug resistance markers(one for resistance to tetracycline, another for resistance toampicillin). You have determined that the restriction enzymes EcoRIand BamHI each cut the plasmid only once while SauIII and RsaI cutthe plasmid twice. You also have determined that the EcoRI andSauIII recognition sites are within the tetracycline resistancemarker, but the BamHI and RsaI recognition sites are not within oreven close to either drug resistance marker. You must use thisparticular vector: a. Which restriction enzyme(s) should you useand why? b. What will happen if you use the BamHI recognition site?What will happen if you use the RsaI recognition site? (Describethis with regards to the functioning of the drug resistance markerand which antibiotic(s) you should use in the media)
