Early gene cloning experiments involved insertion at one
restriction site in the vector; for example, the...
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Biology
Early gene cloning experiments involved insertion at onerestriction site in the vector; for example, the insert would haveand EcoRI site at each end, and he vector would be opened at an RIsite prior to litigation. Under what circumstances would asymmetriccloning be desirable, with the insert having a different site ateach end?
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If the gene is too big and hard to ligate into the plasmid Meaning if the effeciency of the cloning is low for any reason and a single enzyme is used to digest the gene and vector it is also
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