ELISA Data Sheet
1) Please record the sample ID and the color present in each ofthe wells of your test strip (either blue or clear).
Well # | Sample ID | Well Color | Test Result (Positive or Negative) |
1 | Positive Control | | |
2 | Positive Control | | |
3 | Positive Control | | |
4 | Negative Control | | |
5 | Negative Control | | |
6 | Negative Control | Â Â Â Â Â Â Â Â | |
7 | T | | |
8 | T | | |
9 | T | | |
10 | J | | |
11 | J | | |
12 | J | | |
2) Which students are positive for the disease antigen?
3) Which classmate(s) appear to be the originalsource(s) of the disease (see the ELISA Trackinglist)? Note that you should be able to narrow it down to twopeople.
4) If a sample showed a negative result, does this mean that theperson does not have the disease? Why mightsomeone have gotten a negative result when they really do have thedisease?
5) If a sample showed a positive result, was the person in\"direct\" contact with one of the originallyinfected students (answer from question #3)? If they werenot, what does this tell you about disease transmission in apopulation?
6) Why were the tests run in triplicate?
