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Explain how one could use the CRISPR system to generate a GFP C-terminal fusion to a...

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Biology

Explain how one could use the CRISPR system to generate a GFPC-terminal fusion to a protein (i.e. GFP should lie downstream ofthe sequences for your protein of interest.) Sketch a diagram tohelp explain your set-up, and be sure to upload an image of yourdrawing with your assignment. Explain your set-up clearly inwriting.


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Gene tagging howeverthe fusion of endogenous genes to foreign DNA sequenceshas remained cumbersome mostly because it requires the design and creation of a homology template that is specific for each gene to be tagged In this template the foreign sequence is generally flanked by homology arms of 5001500bp on either side that are specific to the targeted endogenous locus Tagging different genes with an identical tagfor example green fluorescent protein GFPwill thus require the design and    See Answer
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