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Explain how to use an ion exchange column containing carboxymethyl resin to separate a protein mixture...

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Biology

Explain how to use an ion exchange column containingcarboxymethyl resin to separate a protein mixture containinginsulin (pl = 5.4), hemoglobin (pl = 7.1) and cytochrome c (pl =10.6).

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Carboxymethyl is a negatively charged molecules and thus is used as cation exchanger because it attract the positive charge So here the mixture contains insulin hemoglobin and cytochrome C isoelectric pH is the point of pH at which the molecules remains in zwitterion mRNAs    See Answer
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