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Explain, in great detail, the protocol of DNA isolation. In your explanation, mention which steps and/or...

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Biology

Explain, in great detail, the protocol of DNA isolation.In your explanation, mention which steps and/or materials areimportant in the process, and explain the significance of thesesteps. Following your explanation, describe where, in the protocol,can contamination occur and cause DNA isolation tofail.

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As per the question below is protocol for EColi plasmidDNA isolation 1 Harvest 5 ml the overnight grown bacterial cells in a centrifuge tube 2 add 100 micro liter solution A or suspension buffer 50 mM glucose 10 mM EDTA pH 8 25 mM Tris pH 8 100 micro gram ml RNase H and dissolve the cells in it and keep on ice 3 Now add 200 microliter of lysis    See Answer
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