Firefly luciferase is the enzyme that allows fireflies toilluminate their abdomens. Because this light generation is anATP-requiring reaction, firefly luciferase can be used to test forthe presence of ATP. In this way, luciferase can test for thepresence of life. The coupled reactions are
1.2.luciferin+O2ATP⇌⇌oxyluciferin+lightAMP+PPiΔG∘=−31.6kJ/mol
If the overall ΔG∘ of the coupled reaction is -5.50 kJ/mol ,what is the equilibrium constant, K, of the first reaction at 23 ∘C?
