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Following dilution of a concentrated solution, the absorbance is determined to be 3.935 on our BioTek...

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Chemistry

Following dilution of a concentrated solution, the absorbance isdetermined to be 3.935 on our BioTek Plate Reader. What, if any,changes should be made to this experiment?

a The sample should be diluted at least two fold

b The sample should be concentrated 2 fold

c The sample should be diluted 100 fold

d The sample should be put into a different microplate.

e There is nothing wrong with this analysis and the 3.935 shouldbe recorded.

Answer & Explanation Solved by verified expert
3.7 Ratings (389 Votes)
According to LambertBeers law absorbance in dependent onconcentration and path length of solution which is represented byformulaA Cl 1where A absorbance Molar absorption coefficientC concentration of solutionl Path length of solutionSoa Whensample is diluted at least two fold    See Answer
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