Following information is from the instruction of Q5 DNA
polymerase: One unit of enzyme will
incorporate 10...
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Biology
Following information is from the instruction of Q5 DNApolymerase: One unit of enzyme willincorporate 10 nmol of dNTP into acid insoluble material in 30minutes at 74°C. The temperature of denaturingshould be (  ) and the temperature of extension shouldbe (  )
94℃ to 98℃ for both.
94℃ to 98℃ for denaturing, 74℃ for extension.
72℃ for denaturing, 50℃ to 65℃ for extension.
94℃ to 98℃ for denaturing, 50℃ to 65℃ for extension.
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3.6 Ratings (544 Votes)
The correct answer is 2nd option ie 94 to 98 for denaturing 74 for extension Reason This initial denaturing temperature is preferably set between 9498 C for most samples The reason behind this is the fact that this
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