Following the grinding of 1.526 g of cereal, extraction ofriboflavin is performed as described in the lab manual, using 40.0mL of water and 0.25 mL of glacial acetic acid, followed bysonication and filtration. 275 uL of this extraction solution isdiluted to 10 mL in pH 7.0 phosphate buffer. Fluorescence intensitymeasured 153.00 a.u. at the operational wavelength. If the externalstandard calibration curve equation (fluorescence intensity v.concentration in ppb) is y = 4.00 *x + 26.7, then what isconcentration of the extraction solution in ppm? How many mg ofriboflavin was extracted?
