For the experimental background, we treated 3T3 mousefibroblasts with EGF and are now trying to detect thephosphorylated tyrosine proteins using primary and secondaryantibodies as stains. We also made 4 different samples (a controlwith no EGF, a sample exposed for 1 minute to EGF, a 5 minute, anda 10 minute).
1) What does the appearance of a new band in a western blotmean?
2) When a band on a western blot increases in density over time,what does it mean?
3) When a band on a western blot decreases in density over time,what does it mean?
4) As it relates to EGF signaling, what process in the cellwould possibly lead a band to increase in density over time?
5) As it relates to EGF signaling, what process in the cellwould possibly lead to a band to decrease in density over time?
6) Why would a control (or 0 time) sample show bands fortyrosine phosphorylated proteins?
Thank you!
