From the bloodstream, siRNA will undergo initial distributionthrough a circulatory system in a few minutes. siRNA undergoescertain pathways to get eliminated from blood. It will getaccumulated in the liver and through renal filtration and finallyit will be excluded through urine. Many delivery systems blocks thepassage of siRNA through kidneys by enfolding in to largerparticles of size 20 nm17. Glomerular basement membrane (GBM) isthe main component of renal filtration barrier. GBM causesdisassembly of catatonic cyclodextrin containing polymer basedsiRNA nanoparticles, thereby facilitating rapid elimination ofsiRNA from circulation. siRNA generally assembled by electrostaticinteractions between catatonic delivery components but that will bedisturbed by GBM ( when siRNA binds with negativity charged GBM)since siRNA normally accumulate in GBM.
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