GATA family of transcription factors are important regulators ofcell identity. The family member GATA1 is required for the switchfrom an immature erythroid cell into a mature red blood cell. Aspart of this switch, GATA1 binds to the consensus DNA sequenceGATAAA through interactions with the DNA major groove. One of theresults is the massive upregulation of globin mRNA.
Design a set of experiments to demonstrate thatGATA1 binds to GATAAA containing sequences anddoes so through major groove recognition/bindingmechanisms.
