Germline mutations occur in sperm or egg and are heritable,whereas somatic mutations occur in non-germ cells and are notheritable. Imagine that you are working in a laboratory which isstudying on tissue/cell specific mutation pattern. You have all thetools (such as cell culture, imaging, sequencing etc.) andmaterials (such as genomic DNA, tissue and primary fibroblast cellsfrom a human individuals). By using a NGS analysis please set upexperiments/pipeline, including which method/algorithm would youuse to map your data and call the somatic and germline mutations.You should focus on the question “How can you distinguish betweengermline and somatic mutations ?†(Please indicate thesteps/methods/algorithms only, No need to indicate thedetails).
