Gradient conditions and methods development a) why is method
development easier with gradient elution chromatography than
isocratic...
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Chemistry
Gradient conditions and methods development a) why is methoddevelopment easier with gradient elution chromatography thanisocratic separations? b) in some detail explain why one would useelevated temperatures in liquid chromatography? c) what is DryLaband why use it?
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a To find answer for this first understand the difference between these two Isocratic Mode of HPLC means the composition of mobile phase remains constant throughout the runIsocratic mode is most commonly used for assay dissolution and routine analysis where you want to separate 2 or 3 compounds in a single run On the other hand in gradient mode composition of mobile phase polarity of mobile phase in varies and its not constant Generally gradient modes
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