How could solubility of an analyte impact its results in HPLC?How would the peak differ, for example? How would a more solublemolecule's peak differ from a less soluble one?
Also, what are the peak areas based on in HPLC? Why is one peaksharper and higher than another, for example? Does that relate tohow well a molecule is retained by the stationary phase in thechromotography?
If you change the detection wavelength, how would the peak areaof the same molecule change?
What does having a larger response factor mean in the internalstandard method in HPLC? does it just relate just to the ratio ofthe two molecules?
