- How does each of your mutations affect the amino acidsequences? Are the mutations missense mutations, silent mutationsor nonsense mutations?
- Point mutation?
- Frameshift-insertion?
- Frameshift-deletion
The non mutated Sequence:
- AUG GAG GUC UUU AAG AGA CAU UUA GAU GUA GCC CUU AGU GAU GUUUAG
Point Mutation:
- AUG GAC GUC UUC AAG AGC CAU UUA GAA GUA GCC CUU AGU GAU GUCUAG
- Translation: Met Asp Val Phe Lys Ser His Leu Glu Val Ala LeuSer Asp Val Stop.
Frameshift Insertion Mutation:
- AUG UGA GGU UUU UAA GUA GAC AUU UAG AUG UAG CCA CUU AGU GAU GUUUAG
- Translated: Met Stop Gly Leu Stop Val Asp Ila Stop Met Stop ProLeu Ser Asp Val Stop
Frameshift Deletion Mutation:
- AUG GAG UUU AAG AGA UUA GAU GUA GCC CUU AGU GAU GUU UAG
- Translated: Met Glu Phe Lys Arg Leu Asp Val Ala Leu Ser Asp ValStop
