How to ensure the label from heavy and light protein was 100%
effective? How do you...
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Biology
How to ensure the label from heavy and light protein was 100%effective? How do you verify the proteins are 100%?
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To determine dyetoprotein molar ratio the extinction molar coefficient of the unlabeled protein must be known Each kind of fluorescent dye molecule absorbs maximally at a particular wavelength to an extent described by its extinction coefficient Amax is the absorbance A of a dye
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