Logo
Home Study Material Contact Search
AI Chat FREE
  1. sciences
  2. chemistry
  3. how would set up a serial dilution to cover a conc

How would set up a serial dilution to cover a concentration range from approximately 21 µM...

90.2K

Verified Solution

Question

Chemistry

How would set up a serial dilution to cover aconcentration range from approximately 21 µM to 120 µM of DHF inanassay (in the well) having a total volume of 200 µL of which 30µL is DHF solution? You are provided a 800 µM DHFstock solution.You need to make a set of DHF dilutions in Eppendorf tubes first.  From these you will take 30 µL eachand dilute intototal volume of 200 µL in the wells

can you plase give me the ansewer step by stepplease

Answer & Explanation Solved by verified expert
4.1 Ratings (557 Votes)
Following is the general idea to solve the problem Stock solution Intermediate solutions in eppendorf tubes Final solutions in wells You are given a stock solution concentration of 800 uM Lets assume that the eppendorf    See Answer
Get Answers to Unlimited Questions

Join us to gain access to millions of questions and expert answers. Enjoy exclusive benefits tailored just for you!

Membership Benefits:
  • Unlimited Question Access with detailed Answers
  • Zin AI - 3 Million Words
  • 10 Dall-E 3 Images
  • 20 Plot Generations
  • Conversation with Dialogue Memory
  • No Ads, Ever!
  • Access to Our Best AI Platform: Flex AI - Your personal assistant for all your inquiries!
Become a Member

Other questions asked by students

Q

ework Transform the polar equation to an equati 5n 6

Basic Math
Q

What type of tool or technique you are suggesting to choose appropriate materials, methods and machines...

Mechanical Engineering
Q

44 Let s start with DNA sequence for the synthesis of protein DNA ATG GGT...

Biology
Q

4 points A Russian scientist named Balyaev was able to tame animal QTL analysis has...

Biology
Q

Find whether the given electric fields are possible or impossible electric field E xyi 2yzj...

Physics
Q

Two wires of equal radii with resistivities p and p2 and length 1 and 2...

Physics
Q

Imagine that a tomato farmer wants to know how many tomatoes she should save this...

Statistics
Q

7 3x 22x 35 A 3x 7 x 5 B 2x 7 x 7 C...

Algebra
Q

Cohen Company produces and sells socks. Variable cost is $7.00 per pair, and fixed costs...

Accounting
Q

The following totals for the month of April were taken from the payroll records of...

Accounting
Q

Melody is a department store manager and wants to examine whether or not female shoppers...

Accounting
Q

Jiminy's Cricket Farm issued a 30-year, 7 percent semiannual bond 3 years ago The bond...

Finance