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How would the yield of a PCR reaction be affected by the following mistakes? Explain your answer. 1....

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Biology

How would the yield of a PCR reaction beaffected by the following mistakes?

Explain your answer.

1. You forgot to add template DNA

2. you added TWICE as much template DNA as is calledfor by the intructions

3. You forgot to add Taq polymerase

4. You add HALF as much Taq polymerase as is calledfor the by instructions

Answer & Explanation Solved by verified expert
4.3 Ratings (625 Votes)
1 If you forget to add template DNA then the primers will hybridize to form primerdimer products It will give rise to nonspecific amplified small fragments 2 If you add TWICE as much template DNA as is called for by the instructions then    See Answer
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