I am trying to isolate the proteins found in the blood of
Alzheimer's patients with the...
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Biology
I am trying to isolate the proteins found in the blood ofAlzheimer's patients with the hope of finding a biomarker that canpotentially indicate the early onset of the disease and waswondering how I could do so? I know centrifugation is an option buthow would I go about doing it specifically?
-my end goal is to simply isolate the proteins from the bloodsamples in order to run ITRAQ analysis on them and MS analysisafter
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ISOLATION OF PROTEINS FROM BLOOD collect the blood sample from the donor and place it on the ice for an hour to allow coagulation of sample To separate the serum from the coagulated blood centrifuge the sample at 2500 rpm 4C for 10 minutes upper layered straw coloured serum is collected in a eppendorf tube it can be stored in 80C for
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