I am trying to purify DNA polymerase from cells DNA polymeraseis a large soluble posivitely charged protein for each purificationstep below select which fractions you will keep in order to end upwith pure DNA polymersase :
1. Cell fractioniation by centrifiguation:
a) Keep Nuclear Pellet
b) Keep mitochondrial Pellet
c) Keep microsomal supernatant
d) Keep microsomal Pellet
2) Salt fractionation with low salt concentration (lowsalt):
a) Keep precipitate
b) Keep supernatant
3) Gel Filtration
a) Keep later fractions
b) Keep middle fractions
c) Keep earlier fractions
4) Cation exchanger:
a) Keep middle fractions
b) Keep later fractions
c) Keep earlier fractions
5) DNA containing affinity column:
a) Later fractions
b) Earlier fractions
