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I have a question about qPCR. How to measure an mRNA level of a gene by qPCR....

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Biology

Ihave a question about qPCR.
How to measure an mRNA level of a gene by qPCR. If I perform arealtive quantification qPCR, first I need to convert mRNA into DNAtemplate by Reverse transcriptase. then I perform a qPCR reaction.For example, comparing with the reference gene, the drug treatmentlead to a decrease in the expression of gene of interest. However,I think that can only determine the gene replication is inhibitedbecause the pcr reaction is DNA replication , not DNAtranscription. So, how can I know the difference in amount of mRNAin a cell?

Answer & Explanation Solved by verified expert
3.6 Ratings (406 Votes)
qPCR is actually used to determine the amount of PCR product of DNA with respect of time Quantitative or semiquantitative RTpcr can be used to determine the expression in mRNA level First you have to convert the mRNA into    See Answer
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