I was analyzing the sample in Agilent HPLC
and I got 3 peaks at 3.654min, 4.540min...
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Chemistry
I was analyzing the sample in Agilent HPLC
and I got 3 peaks at 3.654min, 4.540min and 7.839min with0.1175, 0.1366 and 0.1571 width values
anyone knows how to calculate N value (#of plates), retentionfactor, selectivity factor and resolution value for thischromatograms?
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N the number of theoretical plates is one index used todetermine the performance and effectiveness of columns and iscalculated using equation 11 where tr retention time and W peak widthThis peak width W is based on the baseline intercepts oftangent lines to a Gaussian peak which is equivalent to the peakwidth at 134 of the peak heightHowever to simplify the calculation and
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