If you want to reproduce the shuttling experiment as performedduring week 3. you will need to purchase Cycloheximide (CHX) fromone of three potential suppliers. CHX has a molar mass of 281.35g/mol and can be dissolved in DMSO, water, or EtOH but withdifferent solubilities. You will need to use CHX at a final workingconcentration of 17 μg/mL in a 150 mm dish with a total mediavolume of 14 mL.
Determine how much of the stock solution purchased from eachcompany you would need (in μL) to add to the media in the 150 mmdish to obtain the final working concentration of CHX. Give youranswers in μL using the scientific notation.
a. Company A supplies CHX dissolved in DMSO at 100 mg/ mL:
Volume needed in μL
b. Company A supplies CHX dissolved in water in 2% (w/v)solution
Volume needed in μL:
c. Company A supplies CHX dissolved in EtOH at 50 mM
Volume needed in μL:
