- Imagine you have a plasmid and you cut it with three differentrestriction enzymes (enzymes 1, 2, 3). You then run these fragmentsthrough a gel and get this separation.
- Label the positive and negative terminals on this gel with a(+) and (-) sign.
- If you were working with a single plasmid, how many cut sitesdid restriction enzyme 1 have? Explain?
- When creating a gel, we stain it with Ethidium Bromide or GelRed. What is the purpose of this? Explain.
2- When separating plasmids from proteins and ribosomes, why doyou add N Buffer and THEN centrifuge? Why not just centrifuge? Whatis the advantage of adding the N Buffer?
- I give you 100mL of a 3 M aqueous glucose stock solution. Ineed you to dilute it to make 100mL of a solution that is 0.03M. Toaccomplish this task, you have 2 additional 100 mL graduatedcylinders and however much distilled water you need. The graduatedcylinders are not really accurate at measuring any volume less than5mL. (10 pts)
- How would you dilute 3M stock solution into 0.03 M solution?Explain strategy.
Show your calculations:Ă‚Â Ă‚Â
