In a FRET experiment, the donor GFP (GFP absorbs at 487 nm,emits at 510 nm) is attached to protein A, and the acceptor YFP(YFP absorbs at 514 nm, emits at 535 nm) is attached to protein B.The value of ro is 10AÌŠ. a. If protein A does not bind to proteinB, fluorescence will be observed at what wavelength b. At pH 6, thedistance between the proteins is 25 AÌŠ. What is the efficiency? c.Increasing the pH from 6 to 12 causes the efficiency to increase to0.9. Calculate the separation distance at pH 12.
