In a typical microbiology laboratory, reasons for no bands from
a gel of a polymerase chain...
90.2K
Verified Solution
Link Copied!
Question
Biology
In a typical microbiology laboratory, reasons for no bands froma gel of a polymerase chain reaction may be due to errors relatingto omission of ingredients in the reaction mix and absence of thetarget sequence in the template DNA. Based on (i) primer problemand (ii) purity/potential contamination of the target sequence,explain the reasons for non-appearance on bands.
Answer & Explanation
Solved by verified expert
3.6 Ratings (589 Votes)
PCR or polymerase chain reaction is the inviitro amplification of DNA sequence The reaction requires Various things to be mixed properly and then the reaction are allowed to run in PCR machine Reason for no bands in the gel may be many like
See Answer
Get Answers to Unlimited Questions
Join us to gain access to millions of questions and expert answers. Enjoy exclusive benefits tailored just for you!
Membership Benefits:
Unlimited Question Access with detailed Answers
Zin AI - 3 Million Words
10 Dall-E 3 Images
20 Plot Generations
Conversation with Dialogue Memory
No Ads, Ever!
Access to Our Best AI Platform: Zin AI - Your personal assistant for all your inquiries!