In order to separate the two dyes bound the column a solution isneeded with a substance that has higher affinity for the columnthan the dyes. Isopropyl alcohol is such a substance. If a solutionof water with 70% isopropyl alcohol is applied all the purple dyeelutes (comes off) the column and the dyes do not separate. In factthis is how students clean the column before reuse of the column.Read your protocol and write down the other two concentrations ofisopropyl alcohol here. Why are we using two differentconcentrations of isopropyl alcohol?
Inorder to seperate the two dyes bound the column in a Sep Pak c18Cartridge during purification a solution is needed with a substancethat has higher affinity for the column than the dyes. Isopropylalcohol is such substance. what other two concentrations ofisopropyl can? and when can we use those two differentconcentrations of Isopropyl alcohol
