In paper chromtography, what is the nature of the attractionsbetween the stationary phase (cellulose) and the moving phase(hydrochloric acid/water/acetone) and the species ( Zn2+, Co2+,Cu2+, Cd2+ , Fe3+, and Ni2+ in two unknownsolutions)Ă‚Â Ă‚Â being separated, in particular what affectsthe Rf values and how?
Note: Filter paper (Whatman #1) serves as thestationary phase, and the mobile phase consists of a mixture ofacetone and 6 M hydrochloric acid. A single spot of the mixture tobe analyzed is applied near one edge of a sheet of filter paper. Aspot of solution containing each of the cations that might be inthe unknown mixture is also placed near the edge of the same sheetfor comparison. The treated strip is then placed in a covered jaror beaker (which acts as a developing chamber) containing a shallowlayer of the solvent mixture (see Figure 3). Since filter paper isvery permeable to the solvent, the solvent begins to rise up thestrip by capillary action. The various spots on the developedchromatogram will be highlighted by treatment with several chemicalreagents to enhance their color. The reagents to be used areammonia, dimethylglyoxime (DMG), and 8–hydroxyquinoline (oxine)
