In reversible terminator sequencing how would the sequencing process be affected if the 3 end...
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In reversible terminator sequencing how would the sequencing process be affected if the 3 end blocking group of each nucleotide were replaced with the 3 H present in the dideoxynucleotides used in Sanger sequencing O The presence of the 3 H would prevent the detection of the added nucleotide because a laser would not be able to excite the fluorescent label O The presence of the 3 H would increase the read length allowing longer stretches of DNA to be sequenced The presence of the 3 H would prevent the addition of any subsequent nucleotides so the sequence for each cluster would end after the first nucleotide addition O The presence of the 3 H would have no effect on sequencing because its function is identical to that of the 3 end blocking group
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