In the species A of crustaceans, Ubx is expressed in segments5-10. In the species B, the Ubx expression has expanded anteriorlyand the gene is expressed in segments 4-10. The cis-regulatoryregions of Ubx from the species A were cloned upstream of the GFPreporter, and transgenic lines containing this construct weregenerated for both species.
a) In the species A, in which segments will you see GFP if theconstruct contains all the regulatory regions sufficient to drivethe Ubx expression?
b) In the species B, in which segments will you expect to seethe GFP expression if the difference between the Ubx expression intwo species is due to cis-regulatory changes?
c) In the species B, in which segments will you expect to seeGFP if the difference between the Ubx expression in two species isdue to changes in trans-regulatory factors?
