- In this experiment, you will set things up again the same wayyou did in the very first, only this time you will addMycobacterium tuberculosis instead of yeast. Graph thedata as above, and answer the questions below.
Time (minutes) | no sugar | glucose | sucrose | maltose | lactose | galactose |
0 | 0 | 0 | 0 | 0 | 0 | 0 |
5 | 0 | 3 | 0 | 0 | 2.8 | 3.1 |
10 | 0 | 6 | 0 | 0 | 5.6 | 6.2 |
15 | 0 | 9 | 0 | 0 | 8.8 | 9.3 |
20 | 0 | 12 | 0 | 0 | 11.5 | 12.5 |
25 | 0 | 15 | 0 | 0 | 13.9 | 15.7 |
30 | 0 | 18 | 0 | 0 | 17.2 | 18.9 |
35 | 0 | 21 | 0 | 0 | 20 | 22 |
40 | 0 | 24 | 0 | 0 | 23.5 | 25 |
45 | 0 | 27 | 0 | 0 | 26.5 | 28.5 |
50 | 0 | 30 | 0 | 0 | 29.5 | 31.6 |
55 | 0 | 33 | 0 | 0 | 32.3 | 34.8 |
60 | 0 | 36 | 0 | 0 | 35.2 | 37.8 |
a) What do you notice immediatelyabout sugar utilization in M. tuberculosis?
b) Consider each sugar carefully, andprovide a possible explanation for each of them. You may want torefer back to the structures of the sugars provided at thebeginning.
