Initial rate data for an enzyme that obeys Michaelis-Mentenkinetics are shown in the following table. When the enzymeconcentration is 3 nmol ml−1, a Lineweaver-Burk plot of this datagives a line with a y-intercept of 0.00426(μmol−1ml s).
| [S] μM | v0 (μmol ml−1 s−1) |
| 320 | 169 |
| 160 | 132 |
| 80.0 | 92.0 |
| 40.0 | 57.2 |
| 20.0 | 32.6 |
| 10.0 | 17.5 |
a. Calculate Kcat for the reaction
b. Calculate Km for the enzyme
cWhen the reactions in part (B) are repeated in the presence of12 μM of an uncompetitive inhibitor, they-intercept of the Lineweaver-Burk plot is 0.352(μmol−1 ml s). Calculate K′I for this inhibitor.
