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Look up the RPL4 gene in Drosophila melanogaster (fruit fly) in Entrez Gene. (a) What is RefSeq...

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Biology

Look up the RPL4 gene in Drosophila melanogaster (fruit fly) inEntrez Gene.

(a) What is RefSeq accession number of the genomicsequence of the gene? What is the RefSeq accession number of thecorresponding mRNA sequence of the gene?

(b) Identify the same gene in Bos taurus (cattle).What is the RefSeq accession number of the genomic DNA sequence ofthe RPL4 gene in this animal? What is the RefSeq accession numberof the corresponding mRNA sequence?

(c) Using the Needleman-Wunsch algorithm, determinewhether the two genomic se- quences of the gene in fly and cow arehomologous. How do you know? Do the same with the correspondingmRNA sequences. Which of the two (genomic or mRNA sequence) Is moreuseful for drawing a conclusion regarding homology? Why does thattype of sequence lead to a higher percent identity? To answer thisquestion use the EMBOSS needle program with default parameters.

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Answer & Explanation Solved by verified expert
4.0 Ratings (704 Votes)
Enter the NCBI website and in the search bar choose Refseq from the dropdown menu and then type Drosophila melanogaster RPL4 A RefSeq database is a collection of curated and annotated sequences of DNA RNA and protein a Under results click refseq 4 link on the left side 4 indicates that there are 4 Refseq resultsFor the gene sequence you would find Drosophila melaongaster chromosome 3R Find the accession number under the title as Accession NT0337773 For the mRNA sequence the result would be titled Drosophila melanogaster ribosomal protein L4 RpL4    See Answer
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