Ni-NTA resin was used to purify His6-tagged fusion protein Y.
What method is used to desorb...
60.1K
Verified Solution
Link Copied!
Question
Biology
Ni-NTA resin was used to purify His6-tagged fusion protein Y.What method is used to desorb the fusion protein Y attached to theNi-NTA resin?
Answer & Explanation
Solved by verified expert
4.3 Ratings (546 Votes)
Recombinant proteins which have histidine tags can be purified using immobilized metal ion chromatography IMAC The Histag can be placed on either the N or Cterminus Optimal binding and therefore purification efficiency is achieved when the Histag is freely accessible to metal ion support Histidine tags have strong affinity for metal ions eg Co2 Ni2 Cu2 and Zn2 One of the first support materials used immobilized iminodiacetic acid which can bind metal ions and allow
See Answer
Get Answers to Unlimited Questions
Join us to gain access to millions of questions and expert answers. Enjoy exclusive benefits tailored just for you!
Membership Benefits:
Unlimited Question Access with detailed Answers
Zin AI - 3 Million Words
10 Dall-E 3 Images
20 Plot Generations
Conversation with Dialogue Memory
No Ads, Ever!
Access to Our Best AI Platform: Flex AI - Your personal assistant for all your inquiries!
