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Papain and pepsin are not the only enzymes that can digest antibodies. Find one example of...

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Biology

  1. Papain and pepsin are not the only enzymes that can digestantibodies. Find one example of another enzyme that can digestantibodies and answer the following. (~150-200 words total)
  1. Describe any similarities or differences in the theoreticalantibody fragments generated using this enzyme as compared topepsin and papain. Use a figure to support your answer.
  2. What results would you expect to see in a haemagglutinationassay and on non-reducing and reducing SDS-PAGE gels afterdigesting rabbit anti-SRBC IgG using this enzyme? Include simplediagrams to illustrate your answer.

Answer & Explanation Solved by verified expert
3.9 Ratings (418 Votes)
Antibodies are powerful tools for protein and moleculardetection and purificationPapain and pepsin are not the onlyenzymes that can digest antibodiesOne example of another enzymethat can digest antibodies is Ficina Ficin digestion Mouse IgG1 fragmentsFicin is a thiol protease that can digest mouse monoclonal IgG1into either Fab2 or Fab fragments depending on theconcentration of cysteine included Ficin will generate Fab2 inthe presence of 4mM cysteine Fab fragments result with ficin inthe presence of 25mM cysteineMouse IgG1 Fab and Fab2 preparation by ficindigestion and fragmentationWhereas digestion of antibodies    See Answer
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