PART A: 137 nmol of an antibody that binds strongly to theantigen protein G is covalently bound to the support of an affinitycolumn with a void volume of 1.95 mL. The association equilibriumconstant (KA) for the binding of protein G to theantibody is 5.19 × 108 M-1 at pH 7.2.Calculate the retention factor for protein G on this column at pH7.2. k=?
PART B: At pH 7.2 and a flow rate of 1.00 mL/min, how manyminutes would it take protein G to elute from this column?tr=? min
PART C: If a pH 3.5 elution buffer is applied to the column,protein G elutes at 3.12 min using a flow rate of 1.00 mL/min.Under these conditions, what is the association equilibriumconstant between protein G and the antibody? KA=?M-1
