PCR;
4. A student is using the following PCR cycle conditions toamplify a 384 bp segment of DNA:
i) incubate at 95oC for 30 seconds;
ii) incubate at 53oC for 30 seconds;
iii) incubate at 72oC for 40 seconds.
(a) Describe what is happening duringeach step (i, ii, iii) of the PCR cycle.
(b) After gel electrophoresis of thePCR products, the students see three bands (212 bp, 288 bp, 866 bp)in addition to the desired 384 bp product. Which PCR step (i, ii,iii) should be modified, and how, to try and amplify only thedesired 384 bp band? Explain.
