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Please answer both questions: 1. A number of different buffers/components are used in the PCR. Explain the...

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Biology

Please answer both questions:

1. A number of different buffers/components are usedin the PCR. Explain the function of each:

DNA plasmid


Primer pair


dNTPs mixture


Taq polymerase

2. In our PCR reaction the optimum annealingtemperature is 56oC. Explain the general factors thathelp determine the optimum annealing temperature of a PCRreaction

Answer & Explanation Solved by verified expert
4.0 Ratings (662 Votes)
1DNA plasmid A target gene inserted into a circular piece of DNA called a plasmid plasmid is introduced into bacteria by a process called transformation and bacteria carrying the plasmid are selected using antibiotics Bacteria with the correct plasmid are used to make more plasmid DNA induced to express the gene and make protein PRIMER PAIR Primers are the strands of DNA or RNA that serve as this ini for the DNA replication process and they are used to demarcate the segment of the DNA template to be amplified In the PCR process two primers are matched to    See Answer
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