PLEASE, I NEED TO DESCRIBE EVERY STEPS REALLY WELL. STEPS TOHANDLE AND PREPARE THE SAMPLE AND THEN, STEPS OF RNAseq.
Sequencing RNA from a single circulating tumour cell:
A research laboratory has isolated a CTC from a patient andwould like a description of how you would process the sample andhow you would sequence all nonâ€coding RNA from the cell.
a) Describe the steps involved in HOW you would prepare the RNAfor RNAseq;
b) HOW RNAseq works.
Also explain WHY each step was done and consider the mostappropriate method for isolating nonâ€coding RNAand removing rRNA from your sample.
