Please illustrate the steps to recombinant cloning of the vector
to express c-Myc-GFP-His6 in mammalian cells...
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Biology
Please illustrate the steps to recombinant cloning of the vectorto express c-Myc-GFP-His6 in mammalian cells with detailed DNAsequences of primers. Please explain why the chosen particularvector was used and why the chosen version of the c-Myc gene wasused.
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ANS The human cmyc protooncogene implicated in the manage of many cell tactics which include cell increase and apoptosis encodes three isoforms which differ in their nterminal region the features of these isoforms have in no way been addressed in vivo here we used drosophila melanogaster to observe their capabilities in a fully integrated device first we installed that the human cmyc protein can rescue lethal mutations of the drosophila myc ortholog dmyc demonstrating the organic relevance of this model then we characterized a brand new lethal
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