Polarimetry Lab calculations:
Isomer | Observed Degree of Rotation(Raw data) |
(+) Carovene | 158.75° |
(-) Carovene | 229.75° |
Blank | 200.75° |
Unknown (X) | 175.25° |
I would like some help to figure out how to do this; I want tomake sure that I am doing it correct. Here is what I have done sofar:
- Find specific rotations for all the isomers:
I believe these are the equations to use:
X observed= X raw -Blank            l= 0.8 dm and c= 0.9600 g/cm3
[X]= X observed / lc
Can someone show me the calculations for all of them,this is what I did for Unknown:
My Unknown (X): I did x observed= 175.25-200.75=-25.5°
[X]= X observed / lc -25.5°/(0.8*0.9600)=-33.20°
- Find the Enantiomer Excess:
The equations our teacher gavewere:
EE= [X] observed /[X]pure * 100
([X]pure can be found by[X]= Xpure/ lc
I don’t know how to find x pure. What even is that,where can I get it from the numbers. After I get EE,
I need to find how much of (-) and (+) is in theenantiomer (the equation given for that is
EE= [X]+ XA +[X]- (1-X+)
[X]+ and [X]- aresupposed to be answers from number 1.
