Positive and Negative Control Questions:
Please complete the following table to describe which bacterialspecies should be used as positive and negative controls for eachtest. The table at the bottom of this page will help youcomplete this task. Thenexplains about
What can you learn about a microbe bydoing the Phenol Red test, how do youconduct the Phenol Red test?
What does a positive Phenol Red test look like?(mention acid formation and gasformation)
What does a negative Phenol Red test looklike?(mention acid formation and gasformation)
| Positive Control Species | Negative Control Species |
| Catalase | | |
| PR-Glucose | | |
| PR- Lactose | | |
| PR- Sucrose | | |
| Oxidase Test | | |
The table below describes the cultures we will have available touse as positive and negative controls.
TEST RESULTS OF AVAILABLE CULTURES: | Catalase | PR- Glucose | PR- Lactose | PR- Sucrose | Oxidase |
Alcaligenes (Note* Alcaligenes does not use carbohydratesin its metabolism) | + | - | - | - | + |
E. coli | + | + | + | Variable | - |
Lactococcus | - | Data Not Available | Data Not Available | Data Not Available | Data Not Available |
Pseudomonas | + | - | - | - | + |
