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  3. preparation of competent cells and transformation:

PREPARATION OF COMPETENT CELLS and TRANSFORMATION: 2a. We will be preparing competent MM294 (E. coli) cells using a...

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Biology

PREPARATION OF COMPETENT CELLS andTRANSFORMATION:

2a. We will be preparing competent MM294 (E.coli) cells using a chemical treatment of CaCl2.What are MM294 cells, why do we use them? Define “competent” cellsand describe why/how CaCl2 makes cells “competent”. Whyis “mid-log” phase important? Support your drawing with a cartoonof what cells “look like” before and after treatment.

2b. What does “heat-shock” do (in the transformationprocedure)?

2c. What is LB? What does “LB” stand for and what is itsrole in cloning? What would happen if LB was NOT on theplates?

Answer & Explanation Solved by verified expert
4.2 Ratings (652 Votes)
2 a MM294 are a strain of E coli which isolate in the lab ofMatthew Meselson thats why it named as MM294 when we clone DNA inMM294 strain it gives good yields of DNACompetent cells are those cells that ready to transform or canaccept DNA from    See Answer
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