PREPARATION OF COMPETENT CELLS andTRANSFORMATION:
2a. We will be preparing competent MM294 (E.coli) cells using a chemical treatment of CaCl2.What are MM294 cells, why do we use them? Define “competent†cellsand describe why/how CaCl2 makes cells “competentâ€. Whyis “mid-log†phase important? Support your drawing with a cartoonof what cells “look like†before and after treatment.
2b. What does “heat-shock†do (in the transformationprocedure)?
2c. What is LB? What does “LB†stand for and what is itsrole in cloning? What would happen if LB was NOT on theplates?
